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    sharly's Avatar
    sharly Posts: 1, Reputation: 1
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    #1

    Jun 18, 2013, 11:12 PM
    transduce rodent primary cells with lentiviral constructs?
    Is it possible to transduce rodent primary cells with lentiviral constructs for stable expression of a protein of interest?
    We would like to express a protein in mouse or rat primary neurons, either in a stable or inducible way. Are there any suppliers that provide ready-to-go lentiviral vectors for rodents?
    capeluse's Avatar
    capeluse Posts: 1, Reputation: 1
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    #2

    Jun 20, 2013, 08:09 PM
    You can always plan to do the same with a wide variety of suppliers present world wide.

    It depends on your experimental mindset whether you would prefer to package your own viral particles using lentiviral packaging systems
    I would personally recommend 3-4 suppliers listed below.

    1) ABM Inc, USA. Www.abmgood.com (Cost effective and chance of getting a ready viral particle is high)

    2) Origene Technologies Inc,
    They have retroviral 2nd generation MMLV backbone vectors that can be packaged using a 2nd generation packaging system. Expression ready and validated.

    3)Open Biosystems, Lafayette
    Lentiviral backbone with 3rd generation vector plasmid. Trans lentiviral packaging system along with the clone.

    4) creative biogene(www.creative-biogene.com),
    Using an innovative platform "CLPS" (Creative Lentiviral Packaging System),Price is their greatest advantage, and the service quality is not worse than the above three companies

    Hope the information helps you.

    Best Wishes

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